基于GWAS后分析策略研究TRAPPC9基因对奶牛产奶性状的遗传效应

doi:10.11843/j.issn.0366-6964.2015.01.008

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (1): 60-68.doi: 10.11843/j.issn.0366-6964.2015.01.008

基于GWAS后分析策略研究TRAPPC9基因对奶牛产奶性状的遗传效应

董易春，刘超，王晓，王雅春，张毅，孙东晓，张胜利，张勤，张沅，俞英^*

（中国农业大学动物科技学院，北京 100193）

收稿日期:2014-04-28 出版日期:2015-01-23 发布日期:2015-01-23
通讯作者: 俞英，副教授，博士生导师，主要从事动物分子抗病育种及表观遗传调控研究，E-mail：yuying@cau.edu.cn
作者简介:董易春（1990-），男，江苏人，硕士生，主要从事动物分子数量遗传学研究，E-mail：dongyichun.me@163.com
基金资助:
国家自然科学基金（31272420）；农业部奶业体系项目（CARS-37-04B）；十二五国家科技支持项目（2011BAD28B02）；“863”重大项目（2008AA101002）；教育部基本科研项目(2011JS006)；长江学者与创新团队发展计划（IRT1191）

Confirming the Genetic Effects of Bovine TRAPPC9 on Milk Production Traits Based on Post-GWAS Strategies

DONG Yi-chun，LIU Chao，WANG Xiao，WANG Ya-chun，ZHANG Yi，SUN Dong-xiao，ZHANG Sheng-li，ZHANG Qin，ZHANG Yuan，YU Ying^*

（College of Animal Science and Technology，China Agricultural University，Beijing 100193，China）

Received:2014-04-28 Online:2015-01-23 Published:2015-01-23

摘要/Abstract

摘要：

本研究基于课题组前期对北京地区中国荷斯坦牛群体产奶性状全基因组关联分析结果，在新的中国荷斯坦牛群中将转运蛋白颗粒复合体9（TRAPPC9）基因作为产奶性状的候选基因进行遗传效应验证。研究利用混池测序寻找SNPs位点，结合前期GWAS结果筛选到的SNPs位点，进而分析这些多态性位点与中国荷斯坦牛产奶性状的相关性。结果发现，SNP1、SNP2位点对乳蛋白率和乳糖率效应均显著（P<0.05）；SNP3位点对乳脂率和乳蛋白率的效应均极显著（P<0.01）；SNP4位点对乳脂率有极显著影响（P<0.01）；SNP5位点对乳脂率和乳糖率的效应显著（P<0.05）。同时发现TRAPPC9基因的表达量对产奶性状也有显著影响（P<0.05），而TRAPPC9基因启动子区DNA甲基化对产奶性状无直接显著影响。该结果进一步表明，TRAPPC9基因可以考虑作为分子遗传标记应用于中国荷斯坦牛产奶性状的标记辅助选择。

Abstract:

Based on the result of the previous genome-wide association study(GWAS) for milk production traits in Beijing Chinese Holstein population，trafficking protein particle complex 9(TRAPPC9) as a novel candidate gene for milk production traits was confirmed in new Chinese Holstein population.Promoter，exon and GWAS’s significant region was amplified and sequenced by screening the DNA pools to select single nucleotide polymorphisms(SNP) and analyze their association with milk production traits of Chinese Holstein population.The association analysis showed that the genotypes of SNP1 had significant effect on protein percentage(PP) and lactose percentge(LP)(P<0.01)；the genotypes of SNP2 had significant effect on PP and LP(P<0.05)；the genotypes of SNP3 had significant effect on fat percent(FP) and PP(P<0.01)；the genotypes of SNP4 had significant effect on FP(P<0.01)；the genotypes of SNP5 had significant effect on FP and LP(P<0.05).The expression of TRAPPC9 also had significant effect on milk production traits，but the DNA methylation of promoter region of TRAPPC9 had no direct effect.In conclusion，TRAPPC9 gene can be considered as a molecular marker applied to Chinese Holstein marker-assisted selection.

中图分类号:

董易春，刘超，王晓，王雅春，张毅，孙东晓，张胜利，张勤，张沅，俞英. 基于GWAS后分析策略研究TRAPPC9基因对奶牛产奶性状的遗传效应[J]. 畜牧兽医学报, 2015, 46(1): 60-68.

DONG Yi-chun，LIU Chao，WANG Xiao，WANG Ya-chun，ZHANG Yi，SUN Dong-xiao，ZHANG Sheng-li，ZHANG Qin，ZHANG Yuan，YU Ying. Confirming the Genetic Effects of Bovine TRAPPC9 on Milk Production Traits Based on Post-GWAS Strategies[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 60-68.

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基于GWAS后分析策略研究TRAPPC9基因对奶牛产奶性状的遗传效应

Confirming the Genetic Effects of Bovine TRAPPC9 on Milk Production Traits Based on Post-GWAS Strategies

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